The assassination of former President Eduardo Frei Montalva was said to be instigated by the right-wing dictatorship of Augusto Pinochet.
A Chilean judge on Friday formally accused six people of assassinating a former president in 1982, marking the second time in a decade he has moved to resolve the murder case.
Judge Alejandro Madrid charged doctor Patricio Silva, chauffeur Luis Becerra and intelligence agent Raul Lillo of poisoning Eduardo Frei Montalva,who governed Chile from 1964 to 1970, in a Santiago clinic in 1982.
Pedro Valdivia, another doctor, was accused of accessory to murder, while physicians Helmar Rosenberg and Sergio Gonzalez were accused of covering up the crime.
Court documents also showed that the assassination had been instigated by the right-wing dictatorship of Augusto Pinochet, Madrid said.
The judge had originally brought the charges against the six in 2009, but they were later dismissed.
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